ADLERSHOFER KOLLOQUIUM Analytik: Multiplexed and multimodal analysis of single cells in nano- and picoliter volumes

Wann:
3. November 2020 um 14:00
2020-11-03T14:00:00+01:00
2020-11-03T14:15:00+01:00
Wo:
digital über WebEx
https://bam-bund.webex.com/webappng/sites/bam-bund/meeting/download/a133c8f5de8349f9ae41bb73cb5d4406?siteurl=bam-bund&MTID=me03682b204e1ad72bd391cfab6aa01fd
Kontakt:
Frau Schaefer, Bundesanstalt für Materialforschung und -prüfung (BAM)

Topic:
Multiplexed and multimodal analysis of single cells in nano- and picoliter volumes
Presenter:
Prof. Dr. Petra S. Dittrich
Department of Biosystems Science and Engineering, ETH Zürich, Switzerland
Chair: Dr. Ute Resch, BAM

Summary:

Microfluidic technology is nowadays an important tool for cell analysis. One of the huge potential lays in the possibility to create small liquid environments of defined and reproducible volumes so that analyses can be performed with unprecedented high sensitivity and reproducibility. We use two microfluidic methods to encapsulate cells, (i) droplet microfluidics and (ii) valve-based systems.In this presentation, the latest developments of these strategies will be presented.
(i) Droplet microfluidics is a particularly powerful method for high throughput screening. Monodisperse aqueous droplets of pico- or nanoliter volume immersed in hydrophobic fluid are formed in a microfluidic device at kHz frequencies.
In the field of single-cell analysis, droplet microfluidics is nowadays a wellapproved method and alternative to cytometry. The tiny volumes of the droplets are particularly beneficial, when secreted compounds are analyzed. Most assays in nL droplets, however, are based on fluorescence spectroscopy, which limits the choice of assays and multiplexing capability. In this presentation, our
advanced platform will be introduced that enables analysis of proteins secreted by few cells by the two complimentary analytical methods, time-lapse
fluorescence spectroscopy and matrix-assisted laser desorption/ionization (MALDI)-MS.
(ii) In our alternative “valve-based” method, cells are captured and encapsulated by donut-shaped valves. Here, we achieve size- or marker-selective capture of individualcells and quantitative analysis of secreted factors by means of immunoassays performed on barcoded beads at extremely low limit of detection as required for single-cell analysis. Examples include the analysis of tumor
cells and circulating tumor cells, isolated on-chip from full blood sample.

When it’s time, join your Webex meeting here.

 

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